三磷酸腺苷合酶或ATP合酶，三磷酸腺苷酶（ATPase）的一种，在这里并特指F类的F_oF₁ATP合酶（F Type F_oF₁ ATP Synthase）。它利用呼吸链产生的质子的电化学势能，通过改变蛋白质的结构来进行ATP的合成。

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ATP synthase (EC3.6.3.14) is an important enzyme that creates the energy currency molecule adenosine triphosphate (ATP). ATP is the most commonly used "energy currency" of cells from most organisms. It is formed from adenosine diphosphate (ADP) and inorganic phosphate (P_i), and needs energy for its formation.