起司堡（英语：Cheeseburger）是指夹有干酪的汉堡，通常是将一般干酪切成片状并放在汉堡排上方而成；不过起司汉堡也可以借由添加不同菜色或者在组合方式上做出改变，例如常常便会另外加上生菜、番茄、洋葱、酸黄瓜（Pickled cucumber）、芥末酱、蛋黄酱、番茄酱或者是盐和黑胡椒等。通常切成片状的干酪夹在刚出炉经过烹调的汉堡排后夹入，在遇到汉堡排的肉后干酪往往会遇热而融化，有时候也会使用磨碎过后的干酪。

一般速食餐厅中普遍使用的是加工干酪（Processed cheese），但也有些起司堡则使用如切达起司、瑞士干酪、马苏里拉起司、蓝干酪、辣杰克起司（Pepper jack cheese）等食材，另外为了配合宗教上的要求也有时候会改使用仿真干酪。

A cheeseburger is a hamburger topped with cheese. Traditionally, the slice of cheese is placed on top of the meat patty, but the burger can include many variations in structure, ingredients, and composition. The term itself is a portmanteau of the words "cheese" and "hamburger." The cheese is usually added to the cooking hamburger patty shortly before the patty is completely cooked which allows the cheese to melt. Cheeseburgers are often served with lettuce, tomato, onion, pickles, mustard, mayonnaise, ketchup, and occasionally bacon.