吴门画派，常简称为吴派，是明代绘画的一个地方风格。由沈周、文征明所创，以苏州（别称吴门）为中心。代表画家有董其昌、陈继儒、周天球、莫是龙、李日华、项圣谟、卞文瑜等。

一般讨论吴派的时候，是与浙派（被认为是院画风格）相对，作为文人画的代称。「吴派」一词自明代末期开始出现，在清代被四王继承，成为中国绘画的主流，影响深远，是中国绘画史中最重要的流派之一。

Wu or Wumen School (Chinese:吴门画派) is the term applied to a group of painters of the Southern School during the Ming period of Chinese history. It was not an academy or educational institution, but rather a group united largely by the artistic theories of its members. Often classified as Literati, scholars, or amateur painters (as opposed to professionals), members idealized the concepts of personalizing works and integrating the artists into the art. A Wu School painting is characterized by inscriptions describing the painting, the date, method, or reason for the work, which is usually seen as a vehicle for personal expression. Shen Zhou (1427–1509) is usually cited as the founder of the Wu School.