A method for the cytochrome C-catalyzed fluorescence determination of artemisinin was developed using pyronine B(PB) as substrate. 在细胞色素C催化下,吡啰红B与青蒿素反应导致荧光降低,细胞色素C与青蒿素的反应为酶-底物模型。
The proposed method is based on the inhibitory effect of resorcinol on the reaction of potassium periodate oxidation of pyronine Y in phosphoric acid medium. 在磷酸介质中,痕量间苯二酚对高碘酸钾氧化吡咯红Y使其荧光猝灭具有抑制作用,据此建立了动力学荧光法测定痕量间苯二酚的新方法。