Los Barrios is a small town and municipality in the south of Spain. It is part of the province of Cádiz, which in turn is part of the Andalusia region. It belongs to the Campo de Gibraltar comarca. The town's name means "the districts" in English.

Although the area is known to have been inhabited since prehistoric times, the town is of relatively recent provenance, having been founded in 1704 by refugees from Gibraltar. After abandoning their homes following Gibraltar's capture by Anglo-Dutch forces during the War of the Spanish Succession, some of the inhabitants of Gibraltar took refuge around the existing hermitage of San Isidro at the confluence of the rivers Guadarranque, Guadacorte and Cañas.