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单词 Homidium bromide
释义

Homidium bromide

中文百科

溴化乙锭 Ethidium bromide

(重定向自Homidium bromide)
溴化乙锭的吸收光谱

溴化乙锭(缩写EtBr,EB)是一种核酸染料,常在琼脂糖凝胶电泳中用于核酸染色。在紫外光的照射下,未与核酸结合的溴化乙锭可被激发出橙红色萤光,在与DNA或双股RNA结合时,萤光强度会增强20倍,使得核酸电泳后的胶片可以辨识核酸的相对位置。在核酸分子中,EB分子插入到两层碱基对之间。溴化乙锭是一种强的诱变剂,可致癌或致畸。

大学生物实验经常会使用此染料,操作时必须要佩戴手套并且尽可能的避免任何接触。

英语百科

Ethidium bromide 溴化乙锭

(重定向自Homidium bromide)
Absorption spectrum of ethidium bromide

Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. It is commonly abbreviated as "EtBr", which is also an abbreviation for bromoethane. When exposed to ultraviolet light, it will fluoresce with an orange colour, intensifying almost 20-fold after binding to DNA. Under the name homidium, it has been commonly used since the 1950s in veterinary medicine to treat trypanosomiasis in cattle, a disease caused by trypanosomes. The high incidence of antibiotic resistance makes this treatment impractical in some areas, where the related isometamidium chloride is used instead. Ethidium bromide may be a mutagen, although this depends on the organism exposed and the circumstances of exposure.

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更新时间:2025/6/18 20:50:06