Denitrification is a microbially facilitated process of nitrate reduction (performed by a large group of heterotrophic facultative anaerobic bacteria) that may ultimately produce molecular nitrogen (N₂) through a series of intermediate gaseous nitrogen oxide products. This respiratory process reduces oxidized forms of nitrogen in response to the oxidation of an electron donor such as organic matter. The preferred nitrogen electron acceptors in order of most to least thermodynamically favorable include nitrate (NO₃), nitrite (NO₂), nitric oxide (NO), nitrous oxide (N₂O) finally resulting in the production of dinitrogen (N₂) completing the nitrogen cycle. Denitrifying microbes require a very low oxygen concentration of less than 10%, as well as organic C for energy. Since denitrification can lower leaching of NO₃ to groundwater, it can be strategically used to treat sewage or animal residues of high nitrogen content. Denitrification allows for the production of N₂O, which is a greenhouse gas that can have a considerable influence on global warming.