Agar diffusion test

In Kirby–Bauer testing, discs containing antibiotics are placed on agar where bacteria are growing, and the antibiotics diffuse out into the agar.
If an antibiotic stops the bacteria from growing, one can see circular areas around the wafers where bacteria have not grown.

Petridishes showing zone of inhibition by various extracts like petroleum ether, chloroform, ethanol and acetone[2]

Kirby–Bauer antibiotic testing (KB testing or disc diffusion antibiotic sensitivity testing) is a test which uses antibiotic-impregnated wafers to test whether bacteria are affected by antibiotics. In this test, wafers containing antibiotics are placed on an agar plate where bacteria have been placed, and the plate is left to incubate. If an antibiotic stops the bacteria from growing or kills the bacteria, there will be an area around the wafer where the bacteria have not grown enough to be visible. This is called a zone of inhibition.

单词	Agar diffusion test
释义	Agar diffusion test 英语百科 Agar diffusion test Kirby–Bauer antibiotic testing (KB testing or disc diffusion antibiotic sensitivity testing) is a test which uses antibiotic-impregnated wafers to test whether bacteria are affected by antibiotics. In this test, wafers containing antibiotics are placed on an agar plate where bacteria have been placed, and the plate is left to incubate. If an antibiotic stops the bacteria from growing or kills the bacteria, there will be an area around the wafer where the bacteria have not grown enough to be visible. This is called a zone of inhibition.
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