Under normal conditions, cholinergic neurons in the brain, synthesize acetylcholine from acetyl coenzyme A (acetyl CoA) and choline, in a reaction catalyzed by an enzyme choline acetyltransferase (CAT).

正常情况下，大脑中的胆碱能神经元从乙酰辅酶A（乙酰辅酶A）和胆碱，由胆碱酶催化的反应乙酰转移酶（CAT）。