CDNA文库 Genomic library
cDNA文库筛选在基因功能研究中的应用较早,然而早期的cDNA文库均为「混合型」(pooled),筛选后的回收鉴定工作非常繁杂。近年来由于人类及其它一些物种的全基因组测序工作的完成,使构建基因组范围「cDNA阵列文库」的可能性大为提高。
单词 | Clone bank |
释义 |
Clone bank
中文百科
CDNA文库 Genomic library(重定向自Clone bank)
cDNA文库筛选在基因功能研究中的应用较早,然而早期的cDNA文库均为「混合型」(pooled),筛选后的回收鉴定工作非常繁杂。近年来由于人类及其它一些物种的全基因组测序工作的完成,使构建基因组范围「cDNA阵列文库」的可能性大为提高。
英语百科
Genomic library CDNA文库(重定向自Clone bank)
![]() ![]() ![]() ![]() A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis. |
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