美国海军作战部长（英语：Chief of Naval Operations，简称CNO）是美国海军军阶最高的军官，是参谋长联席会议成员之一。海军作战部长受海军部长的直接领导，负责指挥、资源利用、海军作战部队的战斗力以及由海军部长分配给海军的其他任务。在海军部长的授权下，海军作战部长还负责联合战斗司令部中海军方面的人事和资源调配。海军作战部长的其他职能还有：按美国法典第10编第5033节履行其职责、接受部长安排的其他任务或者将职责与任务分配给其下属军官。与其他参联会成员一样，海军作战部长只是一个行政职务，没有对美国海军部队的军事指挥权。

The Chief of Naval Operations (CNO) is a statutory office (10 U.S.C. § 5033) held by a four-star admiral in the United States Navy, and is the most senior naval officer assigned to serve in the Department of the Navy. The office is a military adviser and deputy to the Secretary of the Navy. In a separate capacity as a member of the Joint Chiefs of Staff (10 U.S.C. § 151) the CNO is a military adviser to the National Security Council, the Homeland Security Council, the Secretary of Defense, and the President. The current Chief of Naval Operations is Admiral John M. Richardson.