沃卓斯基姐妹（英语：The Wachowskis），前身沃卓斯基兄弟（Wachowski Brothers），是人们对美国著名导演拉娜·沃卓斯基（ Lana Wachowski，变性之前叫劳伦斯“拉里”·沃卓斯基 Laurence "Larry" Wachowski）与莉莉·沃卓斯基（Lilly Wachowski，变性之前叫安德鲁·保罗“安迪”沃卓斯基 Andrew Paul "Andy" Wachowski）的称呼。这是因为她们往往由两人合作担当电影作品的导演、编剧以及制片等。其中她们共同导演的《黑客帝国》三部曲已经成为科幻电影的经典作品。她们均是公开的跨性别女性。

Lana Wachowski (formerly Laurence "Larry" Wachowski, born June 21, 1965) and Lilly Wachowski (formerly Andrew Paul "Andy" Wachowski, born December 29, 1967) are sibling American film directors, screenwriters, and producers. They are both openly transgender women. Known together professionally as The Wachowskis and formerly as The Wachowski Brothers, the pair made their directing debut in 1996 with Bound, and reached fame with their second film The Matrix (1999), a major box office success for which they won the Saturn Award for Best Director. They wrote and directed its two sequels: The Matrix Reloaded and The Matrix Revolutions (both in 2003), and were deeply involved in the writing and production of other works in the franchise.