Villanueva de la Serena is a city in the Province of Badajoz, Extremadura, Spain. It has a population of 26,111 (2010) and forms part of a larger urban area with the neighbouring town of Don Benito.

Founded in the thirteenth century as Aldeanueva de Medellín, the city was an important centre for the Mesta in the Middle Ages. It was the birthplace of Pedro de Valdivia who conquered Chile for the Spanish crown.