维斯塔贞女或称护火贞女（拉丁语：virgo Vestalis，原正式全衔：sacerdos Vestalis），是古罗马炉灶和家庭女神维斯塔的女祭司。

维斯塔贞女的祭司职位是由六位女祭司（上古晚期为七位）组成，其年龄介于六至十岁间，必须守贞、侍奉神祇至少三十年。她们的主要任务是守护维斯塔神庙的炉灶，不让圣火浇熄，并以宁芙埃格里娅（Egeria）的圣春之水涤洗神庙。她也负责事先烤制「mola salsa」（意为「盐粉」，粗艾麦粉〔emmer flour，有芒小麦的一种〕混盐水）和「suffimen」（未出生小牛的骨灰），以供特定仪式使用。

In ancient Rome, the Vestals or Vestal Virgins (Vestales, singular Vestalis) were priestesses of Vesta, goddess of the hearth. The College of the Vestals and its well-being was regarded as fundamental to the continuance and security of Rome. They cultivated the sacred fire that was not allowed to go out. The Vestals were freed of the usual social obligations to marry and bear children, and took a vow of chastity in order to devote themselves to the study and correct observance of state rituals that were off-limits to the male colleges of priests.