梵蒂冈宗座图书馆（拉丁语： Bibliotheca Apostolica Vaticana）是圣座的官方图书馆，一般简称为梵蒂冈图书馆，创建于1475年，位于梵蒂冈城的梵蒂冈博物馆旁。其为世界上手抄本收藏最丰富的图书馆之一。梵蒂冈每年准许4000到5000位学者入馆研究（需有大学或研究机构证明，且研究内容水平不得低于研究生论文水平），但只有教皇可以把藏书带出图书馆，而且阅览室严禁携带食物和笔，连矿泉水也不得携入。目前图书馆藏书160万册，其中7.5万册是手抄本，珍贵馆藏包括一部公元350年的圣经，就是所谓“梵蒂冈抄本”,是已知年代最久的圣经；还有一部用1.5公斤黄金装饰书页的圣经。梵蒂冈图书馆藏书包括一些中国古代图书。中华书局出版过《梵蒂冈图书馆所藏汉籍目录》（ISBN 978-7-101-05039-4）。

The Vatican Apostolic Library (Latin:Bibliotheca Apostolica Vaticana), more commonly called the Vatican Library or simply the Vat, is the library of the Holy See, located in Vatican City. Formally established in 1475, although it is much older, it is one of the oldest libraries in the world and contains one of the most significant collections of historical texts. It currently has 75,000 codices from throughout history, as well as 1.1 million printed books, which include some 8,500 incunabula.