用户界面（User Interface，简称UI，亦称用户界面）是系统和用户之间进行交互和信息交换的媒介，它实现信息的内部形式与人类可以接受形式之间的转换。

用户界面是介于用户与硬件而设计彼此之间交互沟通相关软件，目的在使得用户能够方便有效率地去操作硬件以达成双向之交互，完成所希望借助硬件完成之工作，用户界面定义广泛，包含了人机交互与图形用户接口，凡参与人类与机械的信息交流的领域都存在着用户界面。

用户和系统之间一般用面向问题的受限自然语言进行交互。目前有系统开始利用多媒体技术开发新一代的用户界面。

The user interface (UI), in the industrial design field of human–machine interaction, is the space where interactions between humans and machines occur. The goal of this interaction is to allow effective operation and control of the machine from the human end, whilst the machine simultaneously feeds back information that aids the operators' decision-making process. Examples of this broad concept of user interfaces include the interactive aspects of computer operating systems, hand tools, heavy machinery operator controls, and process controls. The design considerations applicable when creating user interfaces are related to or involve such disciplines as ergonomics and psychology.