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单词 Two Dimensional Gel Electrophoresis
释义

Two Dimensional Gel Electrophoresis

中文百科

双向电泳 Two-dimensional gel electrophoresis

(重定向自Two Dimensional Gel Electrophoresis)
双向电泳使用的凝胶
实验室中使用机器将电泳后的蛋白质印渍从凝胶上分离
两次双向电泳的结果,第一次以橘色显示,第二次则以蓝色显示
两次双向电泳的结果,第一次以橘色显示,第二次则以蓝色显示。两次都有显示的信号为黑色,橘色信号只有在第一次电泳结果出现(或第一次远大于第二次),蓝色信号则只有在第二次电泳出现(或第二次远大于第一次)

双向[凝胶]电泳英语:Two-dimensional [gel] electrophoresis)是一种等电聚焦电泳与SDS-PAGE相结合,分辨率更高的蛋白质电泳检测技术。双向电泳后的凝胶经染色蛋白呈现二维分布图,水平方向反映出蛋白在等电点上的差异,而垂直方向反映出它们在分子量上的差别。所以双向电泳可以将分子量相同而等电点不同的蛋白质以及等电点相同而分子量不同的蛋白质分开。双向电泳是快速成长的蛋白质组学技术中最流行最通用的蛋白质分离方法。目前2D-PAGE能够在同一块凝胶上同步检测和定量数千个蛋白质。

英语百科

Two-dimensional gel electrophoresis 双向电泳

(重定向自Two Dimensional Gel Electrophoresis)
2D-Gels (Coomassie stained)
Robots are used for the isolation of protein spots from 2D gels in modern laboratories.
Warping: Images of two 2D electrophoresis gels, overlaid with Delta2D. First image is colored in orange, second one colored in blue. Due to running differences, corresponding spots do not overlap.
Warping: Images of two 2D electrophoresis gels after warping. First image is colored in orange, second one colored in blue. Corresponding spots overlap after warping. Common spots are colored black, orange spots are only present (or much stronger) on the first image, blue spots are only present (or much stronger) on the second image.

Two-dimensional gel electrophoresis, abbreviated as 2-DE or 2-D electrophoresis, is a form of gel electrophoresis commonly used to analyze proteins. Mixtures of proteins are separated by two properties in two dimensions on 2D gels. 2-DE was first independently introduced by O'Farrell and Klose in 1975.

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更新时间:2025/6/18 19:07:10