在音乐记谱中，三十二分音符是一种音符时值。在五线谱记法中，三十二分音符表示为一个实心的椭圆符头加上一个带三条旗帜状的符尾的符杆（如右图的记号）。当三十二分音符与其它有符尾的音符连写时，可以共用符尾（也是一般的情形），这时旗帜状的符尾变为三根连接用的短线。当符头位于五线谱第三在线方（包括第三线）时，符干朝下，否则符干朝上，符干改变方向时，符头的左右方向也要作相应改变（见图）。符尾的上下方向也改变，但始终在符干右边（见图）。三十二分音符的音长是全音符的三十二分之一，即4/4拍中的八分之一拍，3/8或6/8拍中的四分之一拍。二分音符的音长是其十六倍，四分音符的音长是其八倍，八分音符的音长是其四倍，六十四分音符的音长是其二分之一。

In music, a 32nd note (American) or demisemiquaver (British) is a note played for 1/32 of the duration of a whole note (or semibreve). It lasts half as long as a 16th note (or semiquaver) and twice as long as a 64th (or hemidemisemiquaver).

32nd notes are notated with an oval, filled-in note head and a straight note stem with three flags or beams. As with all notes with stems, 32nd notes are drawn with stems to the right of the notehead, facing up, when they are below the middle line of the musical staff. When they are on or above the middle line, they are drawn with stems on the left of the note head, facing down. Flags are always on the right side of the stem, and curve to the right. On stems facing up, the flags start at the top and curve down; for downward facing stems, the flags start at the bottom of the stem and curve up. When multiple 32nd notes or eighth notes (or 16ths, etc.) are next to each other, the flags may be connected with a beam. Similar rules apply to smaller divisions such as 64th notes.