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单词 Thioglycolate broth
释义

Thioglycolate broth

中文百科

巯基乙酸肉汤

瓶内装的就是巯基乙酸肉汤。瓶内的棉签曾经在外阴部蘸过,放在瓶里的目的是检查该部分是否被厌氧细菌感染。[1]
Aerobic and anaerobic bacteria can be identified by growing them in test tubes of thioglycolate broth:  1: Obligate aerobes need oxygen because they cannot ferment or respire anaerobically. They gather at the top of the tube where the oxygen concentration is highest.  2: Obligate anaerobes are poisoned by oxygen, so they gather at the bottom of the tube where the oxygen concentration is lowest.  3: Facultative anaerobes can grow with or without oxygen because they can metabolise energy aerobically or anaerobically. They gather mostly at the top because aerobic respiration generates more ATP than either fermentation or anaerobic respiration.  4: Microaerophiles need oxygen because they cannot ferment or respire anaerobically. However, they are poisoned by high concentrations of oxygen. They gather in the upper part of the test tube, but not the very top.  5: Aerotolerant organisms do not require oxygen as they metabolise energy anaerobically. Unlike obligate anaerobes, though, they are not poisoned by oxygen.  They can be found evenly spread throughout the test tube.

巯基乙酸肉汤是一种多用途、营养丰富的鉴别培养基。其主要作用是测试微生物对氧含量需求的高低。巯基乙酸酯钠在培养基中消耗氧气,从而使专性厌氧微生物得以生长。因为巯基乙酸酯钠与从上部往下扩散的氧气结合,所以氧气浓度随深度的增加而降低。

英语百科

Thioglycolate broth 巯基乙酸肉汤

Aerobic and anaerobic bacteria can be identified by growing them in test tubes of thioglycolate broth:  1: Obligate aerobes need oxygen because they cannot ferment or respire anaerobically. They gather at the top of the tube where the oxygen concentration is highest.  2: Obligate anaerobes are poisoned by oxygen, so they gather at the bottom of the tube where the oxygen concentration is lowest.  3: Facultative anaerobes can grow with or without oxygen because they can metabolise energy aerobically or anaerobically. They gather mostly at the top because aerobic respiration generates more ATP than either fermentation or anaerobic respiration.  4: Microaerophiles need oxygen because they cannot ferment or respire anaerobically. However, they are poisoned by high concentrations of oxygen. They gather in the upper part of the test tube, but not the very top.  5: Aerotolerant organisms do not require oxygen as they metabolise energy anaerobically. Unlike obligate anaerobes, though, they are not poisoned by oxygen.  They can be found evenly spread throughout the test tube.
Thioglycolate broth medium is recommended to isolate strict anaerobes should an anaerobic infection be suspected.[2]

Thioglycolate broth is a multipurpose, enriched, differential medium used primarily to determine the oxygen requirements of microorganisms. Sodium thioglycolate in the medium consumes oxygen and permits the growth of obligate anaerobes. This, combined with the diffusion of oxygen from the top of the broth, produces a range of oxygen concentrations in the medium along its depth. The oxygen concentration at a given level is indicated by a redox-sensitive dye such as resazurine that turns pink in the presence of oxygen.

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更新时间:2025/6/20 15:52:03