温度梯度胶体电泳(temperature gradient gel electrophoresis, TGGE)是电泳技术的一种，通过物质在不同温度下性质的区别进行分离。

TGGE是一种有效的分离DNA、RNA或者蛋白的手段。它利用了不同分子在温度改变下构象的差别进行分离。另外一种类似的技术是变性梯度胶体电泳(denaturing gradient gel electrophoresis, DGGE)，则是利用不同分子在不同浓度的变性剂的作用下失活实现的。

Temperature Gradient Gel Electrophoresis (TGGE) and Denaturing Gradient Gel Electrophoresis (DGGE) are forms of electrophoresis which use either a temperature or chemical gradient to denature the sample as it moves across an acrylamide gel. TGGE and DGGE can be applied to nucleic acids such as DNA and RNA, and (less commonly) proteins. TGGE relies on temperature dependent changes in structure to separate nucleic acids. DGGE was the original technique, and TGGE a refinement of it.