To remove bound CoA from the protein, the protein solution was buffer exchanged into 10 mM citrate (pH 5.0)–1 mM TCEP by using an S1Y10 spiral cartridge (Millipore).
删除约束，农委会从蛋白质，蛋白质的解决办法是缓冲交换到10毫米，柠檬酸（ pH值5.0 ） -1毫米tcep使用s1y10螺旋墨盒（ millipore ） 。
TCEP
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TCEP
TCEP (tris(2-carboxyethyl)phosphine) is a reducing agent frequently used in biochemistry and molecular biology applications. It is often prepared and used as a hydrochloride salt (TCEP-HCl) with a molecular weight of 286.65 gram/mol. It is soluble in water and available as a stabilized solution at neutral pH and immobilized onto an agarose support to facilitate removal of the reducing agent.
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