During the annealing step, we cool everything down to around 55 degrees Celsius, and this allows the primers to bind to their complementary sequences on the single stranded DNA.

在退火的过程中，我们将所有的成分冷却到55摄氏度左右，这使得引物能够与单链DNA上的互补序列结合。

Basically, researchers could prep the sample, add all the necessary ingredients, and then spike it with a piece of single stranded DNA or RNA that has a fluorescent reporter system attached to it.

基本上，研究人员可以准备样品，添加所有必要的成分，然后用附着有荧光报告系统的单链 DNA 或 RNA 对它进行标记。