Single-base extension

Single-base extension (SBE) is a method for determining the identity of a nucleotide base at a specific position along a nucleic acid. The method is used to identify a single-nucleotide polymorphism (SNP).

In the method, an oligonucleotide primer hybridizes to a complementary region along the nucleic acid, to form a duplex, with the primer’s terminal 3’ end directly adjacent to the nucleotide base to be identified. The oligonucleotide primer is enzymatically extended by a single base in the presence of all four nucleotide terminators; the nucleotide terminator complementary to the base in the template being interrogated is incorporated and identified. The presence of all four terminators ensures that no further extension occurs beyond the single incorporated base. Many approaches can be taken for determining the identity of a terminator, including fluorescence labeling, mass labeling for mass spectrometry, measuring enzyme activity using a protein moiety, and isotope labeling.

单词	Single base
释义	Single base 原声例句 Reel知识卷轴 This does not require changing every single base pair in the genome to mammoth. 这不需要将基因组中的每一个碱基对都改变为猛犸象。 VOA常速英语_欧洲 President Macron's government wants to reconcile myriad special pension schemes into a single point based system. 马克龙总统的政府希望将无数的特殊养老金计划协调成一个基于单一点的体系。魔鬼经济学 This is a national representative sample of singles based on the U.S. Census. 这是根据美国人口普查得出的具有全国代表性的单身样本。经济学人 Science and technology To extract information from these fragments, researchers must identify or " sequence" every single base they can lay their hands on. 为了从这些片段中提取信息，研究人员必须识别或“测序” 他们能够接触到的每一个碱基。社会学 Crash Course He went so far as to suggest that deviants could be singled out based on physical characteristics, like a low forehead, stocky build, and prominent jaw and cheekbones, all of which he saw as reminiscent of our primate cousins. 他甚至建议可以根据身体特征挑选出异常者，例如低额头、粗壮身材、突出的下巴和颧骨，他认为所有这些都让人想起我们的灵长类动物表亲。英语百科 Single-base extension Single-base extension (SBE) is a method for determining the identity of a nucleotide base at a specific position along a nucleic acid. The method is used to identify a single-nucleotide polymorphism (SNP). In the method, an oligonucleotide primer hybridizes to a complementary region along the nucleic acid, to form a duplex, with the primer’s terminal 3’ end directly adjacent to the nucleotide base to be identified. The oligonucleotide primer is enzymatically extended by a single base in the presence of all four nucleotide terminators; the nucleotide terminator complementary to the base in the template being interrogated is incorporated and identified. The presence of all four terminators ensures that no further extension occurs beyond the single incorporated base. Many approaches can be taken for determining the identity of a terminator, including fluorescence labeling, mass labeling for mass spectrometry, measuring enzyme activity using a protein moiety, and isotope labeling.
随便看	cross ourselves的意思 Crossout的意思 cross ... out的意思 cross out的意思 crossover的意思 Cross Over的意思 cross over analysis的意思 crossover area的意思 crossover assembly的意思 crossover bend的意思 cross over bend的意思 crossover bleed的意思 crossover block的意思 cross over bush的意思 crossover bush的意思 Crossover cable的意思 crossover chromatid的意思 crossover circuit的意思 crossover clamp的意思 crossover coil的意思 cross over coil的意思 crossover comparison的意思 crossover connection的意思 crossover correction unit的意思 crossover critical exponent的意思

Single base

Single-base extension