High-performance liquid chromatography

Schematic representation of an HPLC unit.
(1) Solvent reservoirs, (2) Solvent degasser, (3) Gradient valve, (4) Mixing vessel for delivery of the mobile phase, (5) High-pressure pump, (6) Switching valve in

$A rotary fraction collector collecting HPLC output. The system is being used to isolate a fraction containing Complex I from E. coli plasma membranes. About 50 litres of bacteria were needed to isolate this amount.[2]$

High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography), is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out the column.

单词	Reversed phase HPLC
释义	Reversed phase HPLC 英语百科 High-performance liquid chromatography $A rotary fraction collector collecting HPLC output. The system is being used to isolate a fraction containing Complex I from E. coli plasma membranes. About 50 litres of bacteria were needed to isolate this amount.[2]$ High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography), is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out the column.
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Reversed phase HPLC

High-performance liquid chromatography