娘子谷大屠杀是纳粹党在第二次世界大战进攻苏联期间，于乌克兰首都基辅附近山沟进行一系列大屠杀。

最臭名昭著的屠杀事件发生在1941年9月29日-30日，33,771名犹太人在单一行动中被杀死。军政府长官少将弗里德里克·埃伯哈特，南方集团军麾下的警察部队总司令弗里德里克·耶克尔恩（Friedrich Jeckeln）和别动队C队司令奥托·拉斯彻（Otto Rasch）决意要消灭基辅内全部的犹太人。特遣队4A士兵、***、保安处、乌克兰警方运行该次屠杀 。娘子谷大屠杀是纳粹政权进攻苏联期间最大的单一大屠杀，只有1943年11月收缴行动（42,000-43,000人受害）的死亡人数更多，1941年敖德萨大屠杀在1941年10月发生，当时罗马尼亚部队杀死超过50,000名犹太人。

Babi Yar (Russian:Бабий Яр, Babiy Yar; Ukrainian:Бабин Яр, Babyn Yar) is a ravine in the Ukrainian capital Kiev and a site of massacres carried out by German forces and local collaborators during their campaign against the Soviet Union.

The most notorious and the best documented of these massacres took place from 29–30 September 1941, wherein 33,771 Jews were killed. The decision to kill all the Jews in Kiev was made by the military governor, Major-General Kurt Eberhard, the Police Commander for Army Group South, SS-Obergruppenführer Friedrich Jeckeln, and the Einsatzgruppe C Commander Otto Rasch. It was carried out by Sonderkommando 4a soldiers, along with the aid of the SD and SS Police Battalions backed by the local police. The massacre was the largest mass killing for which the Nazi regime and its collaborators were responsible during its campaign against the Soviet Union and is considered to be "the largest single massacre in the history of the Holocaust" to that particular date, surpassed only by Aktion Erntefest of November 1943 in occupied Poland with 42,000–43,000 victims and the 1941 Odessa massacre of more than 50,000 Jews in October 1941, committed by Romanian troops.