浦金氏纤维（英语：Purkinje fibers）位于心脏的心室内壁，就在心内膜（endocardium）之下。这些纤维是特化的心肌细胞，可传导生理上的电位信号并刺激心脏以协调的方式收缩运作。

The Purkinje fibers (/pərˈkɪndʒiː/ pər-KIN-jee) (Purkinje tissue or subendocardial branches) are located in the inner ventricular walls of the heart, just beneath the endocardium in a space called the subendocardium. The Purkinje fibers are specialised conducting fibers larger than cardiomyocytes with fewer myofibrils and a large number of mitochondria that are able to conduct cardiac action potentials more quickly and efficiently than any other cells in the heart. Purkinje fibers allow the heart's conduction system to create synchronized contractions of its ventricles, and are, therefore, essential for maintaining a consistent heart rhythm.