Hook effect
（重定向自Prezone）
![Illustration of hook effect adapted from Schiettecatte et al.[1]](/uploads/202502/04/Hook_effect4152.png)
The hook effect or the prozone effect is a type of interference which plagues certain immunoassays and nephelometric assays, resulting in false negatives or inaccurately low results. Other common forms of interference include antibody interference, cross-reactivity and signal interference. The phenomenon is caused by very high concentrations of a particular analyte or antibody and is most prevalent in one-step (sandwich) immunoassays.