Plaque hybridization

Plaque hybridization is a technique used in Molecular biology for the identification of recombinant phages. The procedure can also be used for the detection of differentially represented repetitive DNA. The technique involves the hybridization of isolated DNA from each of the thousands of the phage λ, from our cDNA to a label probe for our gene of study. This is followed by autoradiography to detect the position of the label. The plaque hybridization procedure is similar to Colony hybridization but have some advantages over the later due to the smaller and well defined area of the filter to which the DNA bounds.

单词	Plaque hybridization
释义	Plaque hybridization 英语百科 Plaque hybridization Plaque hybridization is a technique used in Molecular biology for the identification of recombinant phages. The procedure can also be used for the detection of differentially represented repetitive DNA. The technique involves the hybridization of isolated DNA from each of the thousands of the phage λ, from our cDNA to a label probe for our gene of study. This is followed by autoradiography to detect the position of the label. The plaque hybridization procedure is similar to Colony hybridization but have some advantages over the later due to the smaller and well defined area of the filter to which the DNA bounds.
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