核苷三磷酸（英语：Nucleoside triphosphate；NTP）是一种含有三个磷酸基团的核苷酸。自然界常见的型态包括腺苷三磷酸（ATP）、鸟苷三磷酸（GTP）、胞苷三磷酸（CTP）、胸腺苷三磷酸（TTP）以及尿苷三磷酸（UTP）等。这些分子中包含一个核糖，若是将核糖替换成去氧核糖，那么会使核苷三磷酸变成去氧核苷三磷酸，写成dNTP，如去氧腺苷三磷酸（dATP）、去氧鸟苷三磷酸（dGTP）等。

A nucleoside triphosphate (NTP) is a molecule containing a nucleoside bound to three phosphate groups. It is thus one type of nucleotide. Nucleotide derivatives are necessary for life, as they are the building blocks of nucleic acids and have thousands of other roles in cell metabolism and regulation. NTPs generally provide energy and phosphate groups for phosphorylation.