游蛇亚科（学名：Natricinae）是蛇亚目游蛇科下的一个亚科，其下目前共有28个属。

The Natricinae are a subfamily of the Colubridae family of snakes, which comprises 28 genera. Members include many very common snake species, such as the European grass snakes, and the North American water snakes and garter snakes. Some members of the subfamily are known as keelbacks, because their dorsal scales exhibit strong keeling.