Micrococcal nuclease treatment indicates that the remodeling of the demembranated sperm chromatin has occurred and the structure of nucleosome is formed during nuclear reconstitution.
同时，利用小球菌核糖核酸酶处理的结果表明，去膜精子在此非细胞体系中发生了结构改建并且在重建过程中形成了核小体结构。
Micrococcal nuclease
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英语百科
Micrococcal nuclease
Micrococcal Nuclease (EC3.1.31.1, S7 Nuclease, MNase, spleen endonuclease, thermonuclease, nuclease T, micrococcal endonuclease, nuclease T', staphylococcal nuclease, spleen phosphodiesterase, Staphylococcus aureus nuclease, Staphylococcus aureus nuclease B, ribonucleate (deoxynucleate) 3'-nucleotidohydrolase) is an endo-exonuclease that preferentially digests single-stranded nucleic acids. The rate of cleavage is 30 times greater at the 5' side of A or T than at G or C and results in the production of mononucleotides and oligonucleotides with terminal 3'-phosphates. The enzyme is also active against double-stranded DNA and RNA and all sequences will be ultimately cleaved.
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