代谢途径（英语：metabolic pathway）在生物化学中，是一连串在细胞内发生的化学反应，并由酶所催化，形成使用或保存的代谢物，或引发另一个代谢途径（称为「流量控制反应」）。多种途径都是精细的，并涉及原来物质逐步修饰成所需的化学结构的化合物。

细胞内不同代谢途径组成了代谢网络。底物是否进入代谢途径，要视乎细胞的需要，即合成代谢物及分解代谢物浓度的独特组合（流量控制反应的动力）。代谢途径包括主要的代学反应（一般都是需要酶的）令生物保持牠的内环境稳态。

In biochemistry, a metabolic pathway is a series of chemical reactions occurring within a cell. The reactants, products, and intermediates of an enzymatic reaction are known as metabolites. In a pathway, the reactant is modified by a sequence of chemical reactions. These reactions are catalyzed by enzymes, where the product of one enzyme acts as the substrate for the next. These enzymes often require dietary minerals, vitamins, and other cofactors to function.