《古腾堡圣经》（Gutenberg Bible，亦称四十二行圣经，即42-line Bible，此外还有Mazarin Bible或B42）是《圣经》拉丁文公认翻译的印刷品，由同名人古腾堡于1454年到1455年在德意志美因兹采用活字印刷术印刷的。这版《圣经》是最著名的古版书，他的产生标志着西方图书批量生产的开始。

完整的拷贝由1282页组成，装订成两卷。

据说一共有180个谷登堡圣经被印刷出来，40本印刷在羊皮纸上，另外140本印刷在纸上。 2003年，已知现存的谷登堡圣经包括11个羊皮纸上的完全拷贝，一个羊皮纸上的新约全书，48个基本完全的纸上的拷贝，还有一些零散的拷贝。

The Gutenberg Bible (also known as the 42-line Bible, the Mazarin Bible or the B42) was the first major book printed using mass-produced movable type. It marked the start of the "Gutenberg Revolution" and the age of the printed book in the West. Widely praised for its high aesthetic and artistic qualities, the book has an iconic status. Written in Latin, the Catholic Gutenberg Bible is an edition of the Vulgate, printed by Johannes Gutenberg, in Mainz, in present-day Germany, in the 1450s. Forty-nine copies, or substantial portions of copies, survive, and they are considered to be among the most valuable books in the world, even though no complete copy has been sold since 1978. The 36-line Bible, believed to be the second printed version of the Bible, is also sometimes referred to as a Gutenberg Bible, but is possibly the work of another printer.