五月花号（英语：Mayflower）是1620年从英格兰的普利茅斯搭载着清教的分支---“分离教派”Pilgrim的一些人前往位于美洲新大陆马萨诸塞普利茅斯殖民地的客船。于9月6日包括35名清教徒在内的共102名乘客登上名叫五月花号的木制帆船，从英格兰出发，11月11日在鳕鱼角附近靠岸（均按儒略历）66天的航程只有一人死去，且诞生了一名婴孩，所以仍然维持102名抵达目的地。这次航行是在1607年由弗吉尼亚公司成功地创建了第一个永久性英国定居点詹姆斯敦的鼓舞下进行的。此次航行的主要领袖是威廉·布拉德福德（William Bradford）和 威廉·布鲁斯特（William Brewster），他们当时用的主要是日内瓦圣经，分别被美国清教徒博物馆（Pilgrim Hall Museum）和海外蒋博士收藏。

The Mayflower was the ship that transported the first English Separatists, known today as the Pilgrims, from Plymouth to the New World in 1620. There were 102 passengers, and the crew is estimated to have been about 30, but the exact number is unknown. This voyage has become an iconic story in some of the earliest annals of American history, with its story of death and of survival in the harsh New England winter environment. The culmination of the voyage in the signing of the Mayflower Compact was an event which established a rudimentary form of democracy, with each member contributing to the welfare of the community.