马尼图林区（英语：Manitoulin District）是加拿大安大略省北部一个分区，覆盖休伦湖中的马尼图林岛及邻近岛屿，北面隔休伦湖的北湖峡与阿尔戈马区和萨德伯里区对望，西面隔水与美国密芝根州契皮瓦县为邻。马尼图林区于1888年脱离阿尔戈马区而成，行政中心设于戈尔湾。据2011年人口普查所示，马尼图林区有13048名居民。马尼图林区基本上没有任何地方行政功能，区内的服务主要由建制乡镇政府、地区服务局或直接由省政府提供。

Manitoulin District is a district in Northeastern Ontario within the Canadian province of Ontario. It was created in 1888 from part of Algoma District. The district seat is Gore Bay.

It comprises Manitoulin Island primarily, as well as a number of smaller islands surrounding it, such as Barrie, Cockburn, and Great La Cloche islands. Previously it included the municipality of Killarney on the mainland, until this was transferred to Sudbury District in the late 1990s. Subsequently, more mainland portions were added to Killarney and these, together with Unorganized Mainland Manitoulin District, were also transferred to Sudbury District in 2006, about 1,600 square kilometres (600 sq mi) in all.