Any blood vessels encountered along the way are ligated.

操作过程中遇到的任何血管都要结扎。

Open, identify, ligate, remove, irrigate, close.

打开，确认，结扎，移出，冲洗，缝合。

Transfer of the ligated plasmid into an E. coli strain (transformation).

7。转化成功的细菌的筛选。

Need a ligating suture on the splenic artery while I make the cut.

在我切开时 得用缝线结扎脾动脉。