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单词 Leupeptin
释义

Leupeptin

英语百科

Leupeptin

Crystal structure of Leupeptin (silver) in the Trypsin (green) binding pocket. Hydrogen bonds are shown as yellow dotted lines. Rendered from PDB 1JRS.

Leupeptin, also known as N-acetyl-L-leucyl-L-leucyl-L-argininal, is a naturally occurring protease inhibitor that can inhibit cysteine, serine and threonine peptidases.

It is often used during in vitro experiments when a specific enzymatic reaction is being studied. When cells are lysed for these studies, proteases, many of which are contained within lysosomes, are released. These proteases, if freely present in the lysate, would destroy any products from the reaction being studied, and make the experiment uninterpretable. For example, leupeptin could be used in a calpain extraction to keep calpain from being hydrolyzed by specific proteases. The suggested concentration is 1-10 µM (0.5-5 µg/ml).

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更新时间:2025/6/17 17:06:39