毒气室，是一种自杀、他杀或者死刑的工具，方法是将自己或者别人、犯人等上锁在一个封闭的空间，施放有毒气体，引发死亡。

如此的处死方式曾经在二次世界大战期间被纳粹德国所使用，目的是集体谋杀。

A gas chamber is an apparatus for killing humans or animals with gas, consisting of a sealed chamber into which a poisonous or asphyxiant gas is introduced. The most commonly used poisonous agent is hydrogen cyanide; carbon dioxide and carbon monoxide have also been used. Gas chambers were used as a method of execution for condemned prisoners in the United States beginning in the 1920s and continues to be a legal execution method in three states. During the Holocaust, large-scale gas chambers designed for mass killing were used by Nazi Germany as part of their genocide program, and also by the Independent State of Croatia at the Jasenovac concentration camp. The use of gas chambers in North Korea has also been reported.