基韦斯特国家野生动物保护区(英语：Key West National Wildlife Refuge)是一座位于美国佛罗里达州门罗县的国家野生动物保护区，处于基韦斯特和干龟群岛之间。保护区内仅有8.171平方公里（2,019英亩）的礁岛土地处于海平面之上。这些岛屿上并没有人生存，它们被指定为国家荒野保护系统的一部份。这个保护区设立的目的是保护和为区内所有野生动物提供栖居地。

此外保护区还拥有767平方公里（189,530英亩）的土地，并为美国海岸警卫队维持着0.623平方公里（154英亩）的土地。同时它还拥有834.822平方公里（206,289英亩）的与佛罗里达相接的海域。

The Key West National Wildlife Refuge is a 189,497 acre (766.867 km) National Wildlife Refuge located in Monroe County, Florida, between Key West, Florida and the Dry Tortugas . Only 2,019 acres (8.171 km) of land are above sea level, on several keys within the refuge. These keys are unpopulated and are also designated as Wilderness within the Florida Keys Wilderness. The refuge was established to provide a preserve and breeding ground for native birds and other wildlife as well as to provide habitat and protection for endangered and threatened fish, wildlife, plants and migratory birds.