Interference reflection microscopy

$Fig. 1: Principle of Interference Reflection Microscopy (IRM) showing interference effect on reflected waves and the result on the final image intensity. Dark purple wave represents the light from the light source. The light purple waves are the reflections from the cell membrane and from the glass surface. Upon hitting the glass surface, the reflected waves are shifted half a wavelength. When the membrane is very close to the glass, the reflected light will be reflected out of phase with the reflected beam from the glass. This will cause interference (see red line), resulting in a dark pixel. If there is more distance between the membrane and the glass, the returning waves will be less shifted and won't cause interference (see red line), resulting in a brighter pixel in the final image. Indicated are the typical refractive indices of the glass, medium and the cell membrane, which determine the amount of reflection.$

Interference reflection microscopy or IRM is an optical microscopy technique that utilizes polarized light to form an image of an object on a glass surface. The intensity of the signal is a measure of proximity of the object to the glass surface. This technique can be used to study events at the cell membrane without the use of a (fluorescent) label in contrast to TIRF microscopy.

单词	Interference reflection microscopy
释义	Interference reflection microscopy 英语百科 Interference reflection microscopy $Fig. 1: Principle of Interference Reflection Microscopy (IRM) showing interference effect on reflected waves and the result on the final image intensity. Dark purple wave represents the light from the light source. The light purple waves are the reflections from the cell membrane and from the glass surface. Upon hitting the glass surface, the reflected waves are shifted half a wavelength. When the membrane is very close to the glass, the reflected light will be reflected out of phase with the reflected beam from the glass. This will cause interference (see red line), resulting in a dark pixel. If there is more distance between the membrane and the glass, the returning waves will be less shifted and won't cause interference (see red line), resulting in a brighter pixel in the final image. Indicated are the typical refractive indices of the glass, medium and the cell membrane, which determine the amount of reflection.$ Interference reflection microscopy or IRM is an optical microscopy technique that utilizes polarized light to form an image of an object on a glass surface. The intensity of the signal is a measure of proximity of the object to the glass surface. This technique can be used to study events at the cell membrane without the use of a (fluorescent) label in contrast to TIRF microscopy.
随便看	unequal ice loading的意思 unequal impact parameter的意思 unequal impulse的意思 unequal interval的意思 unequal intervals的意思 unequal irradiation effect的意思 unequalised的意思 unequality的意思 unequalize的意思 unequalize的意思 unequalized profit rate of capital的意思 unequal的意思 unequalled的意思 unequalled strength的意思 unequal leg angle的意思 unequal leg fillet welds的意思 unequal liability的意思 unequal loading的意思 unequally的意思 unequally accurate measurement的意思 unequally distributed load的意思 unequally pinnate的意思 unequally spaced intervals的意思 unequally spaced scale的意思 unequally spaced teeth的意思