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单词 Hoechst 33258
释义

Hoechst 33258

英语例句库

The former Hoechst headquarters, a redbrick relic of the 1920s, is an admired piece of Bauhaus architecture.

一座1920年代红砖建筑,作为前赫斯特的总部,是包豪斯建筑学派的经典之作。

中文百科

赫斯特染色 Hoechst stain

(重定向自Hoechst 33258)
DNA经过蓝色33258赫斯特染色的HeLa细胞,中间与右边的细胞正经历分裂间期,可见整个细胞核都呈蓝色。而左方的细胞正在进行有丝分裂,其细胞核正在崩解,准备接下来要进行的分裂。
Hoechst 33342
Hoechst 33258 (magenta) unido al surco menor del ADN (verde y azul). Imagen de PDB 264D .
Chemical structure of Hoechst dyes.

赫斯特染色(英语:Hoechst stains)是萤光染色中的一种类型,专门用来在萤光显微观察下标示DNA。由于可将DNA标示,此种染色方式也可以用来辨识内含DNA的细胞核及粒线体的位置。

常用的两种染剂为Hoechst 33258Hoechst 33342 ,这两种染剂可在波长350奈米的紫外光下激化。不过赫斯特染剂是潜在的突变原,处置上需要较为小心。

英语百科

Hoechst stain 赫斯特染色

(重定向自Hoechst 33258)
Chemical structure of Hoechst dyes.
Excitation/Emission spectra of Hoechst dyes.
Hoechst 33258 (magenta) bound to the minor groove of DNA (green and blue). From PDB: 264D​.
Transmission image of HeLa cells, with overlay of Hoechst 33258 staining (blue). 
The leftmost cell is in the prometaphase stage of mitosis; its chromosomes fluoresce brightly because they contain highly compacted DNA.

Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similar excitation/emission spectra.

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更新时间:2025/6/17 10:13:18