在基督教中，大使命（英语：Great Commission）是指耶稣复活后对门徒指示，将其教导传到世界各地。在基督教神学中，这使命是基督徒传教士活动的一个主要依据。

在各版本《四福音书》和《使徒行传》中，对大使命有着不同的记载 ，而所有有关大使命记载都是描述在耶稣复活后发生的。

最常为人引用的章节为马太福音第28章第16-20节：

In Christianity, the Great Commission is the instruction of the resurrected Jesus Christ to his disciples that they spread his teachings to all the nations of the world. The most famous version of the Great Commission is in Matthew 28:18–20, where on a mountain in Galilee Jesus calls on his followers to baptize all nations in the name of the Father, the Son, and the Holy Spirit.