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单词 Genomic library
释义

Genomic library

原声例句
2017级专外有声

Genomic libraries are prepared from random fragments of genomic DNA.

然而,基因组文库作为寻找一种特定基因的方法效率并不高,尤其对于含有大量非编码DNA的真核基因组中。

2017级专外有声

However, genomic libraries may be an inefficient method of finding a gene, particularly in large eukaryotic genomes, where much of the DNA is noncoding.

替代的方法是利用已知表达目的基因的细胞或组织来源的mRNA所构建的文库。

中文百科

CDNA文库

cDNA文库筛选在基因功能研究中的应用较早,然而早期的cDNA文库均为「混合型」(pooled),筛选后的回收鉴定工作非常繁杂。近年来由于人类及其它一些物种的全基因组测序工作的完成,使构建基因组范围「cDNA阵列文库」的可能性大为提高。

英语百科

Genomic library CDNA文库

Genomic Library Construction
Colony Blot Hybridization
bac vector
Whole genome shotgun sequencing versus Hierarchical shotgun sequencing

A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.

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更新时间:2025/6/17 16:48:48