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单词 Fluorescence quenching
释义

Fluorescence quenching

英语例句库

Through studies on the fluorescence quenching of P3’–P6’ by Rubredoxin, the extent of quenching was found to be stronger in the polymer with more typical features of helix conformation.

10. 研究了P3’–P6’ 对铁硫蛋白Rubredoxin的荧光猝灭效应,发现螺旋形构象特征越典型的聚合物被猝灭的的效率越高。

英语百科

Quenching (fluorescence)

(重定向自Fluorescence quenching)
Two samples of quinine dissolved in water with a violet laser (left) illuminating both. Typically quinine fluoresces blue, visible in the right sample. The left sample contains chloride ions which quenches quinine's fluorescence, so the left sample does not fluoresce visibly (the violet light is just scattered laser light).
Donor emission and quencher absorption spectral overlap
Comparison of static and dynamic quenching mechanisms

Quenching refers to any process which decreases the fluorescence intensity of a given substance. A variety of processes can result in quenching, such as excited state reactions, energy transfer, complex-formation and collisional quenching. As a consequence, quenching is often heavily dependent on pressure and temperature. Molecular oxygen, iodide ions and acrylamide are common chemical quenchers. The chloride ion is a well known quencher for quinine fluorescence. Quenching poses a problem for non-instant spectroscopic methods, such as laser-induced fluorescence.

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更新时间:2025/6/21 4:49:26