文明化五部族（英语：Five Civilized Tribes），指美国原住民中的五个部族。分别是奇克索人、切罗基人、乔克托人、克里克人以及塞米诺尔人。有时为了避免暗指其他族群较「野蛮」，「文明化五部族」一词会以「五部族」来取代。

这五个部族吸取了较多的欧洲移民的生活习惯，且与邻近族群的关系较为良善。早期生活在美国东南部；后来在联邦政府的政策下，被向西安置在密西西比河以西地区一处称做「印地安领地」（Indian Territory）的地方，也就是今天的奥克拉荷马州东部。其中一场迁徙发生在马丁·范·贝伦总统所推行的法案实施之后，又称1838年的「血泪之路」（Trail of Tears）。

The term "Five Civilized Tribes" derives from the colonial and early federal period. It refers to five Native American nations—the Cherokee, Chickasaw, Choctaw, Creek (Muscogee), and Seminole. These are the first five tribes that Anglo-European settlers generally considered to be "civilized" according to their own world view, because these five tribes adopted attributes of the colonists' culture, for example, Christianity, centralized governments, literacy, market participation, written constitutions, intermarriage with white Americans, and plantation slavery practices. The Five Civilized Tribes tended to maintain stable political relations with the Europeans.