伊甸园（希伯来语：גן עדן），根据《圣经·创世记》记载，耶和华上帝照自己的形像造了人类的袓先，男的称亚当，女的称夏娃，安置第一对男女住在伊甸园中。伊甸园在圣经的原文中含有快乐，愉快的园子的意思（或称乐园）。《圣经》记载伊甸园在东方，有四条河从伊甸流出滋润园子。这四条河分别是幼发拉底河、底格里斯河、基训河和比逊河。现存的只有前两条。

上帝的原本旨意是要他们生儿育女，遍满地面，使整个地球都成为伊甸园。但后来夏娃受撒旦（蛇）的哄诱，偷食了知善恶树所结的果子，也让亚当食用，上帝知道后，遂将二人逐出伊甸园，又在伊甸园东边安设基路伯（天使）和发出火焰转动的剑，把守生命树的道路。

The Garden of Eden (Hebrew גַּן עֵדֶן, Gan ʿEḏen) is the biblical "garden of God", described most notably in the Book of Genesis chapters 2 and 3, and also in the Book of Ezekiel. The "garden of God", not called Eden, is mentioned in Genesis 13, and the "trees of the garden" are mentioned in Ezekiel 31. The Book of Zechariah and the Book of Psalms also refer to trees and water in relation to the temple without explicitly mentioning Eden.